14
HTB-94 SW 1353 人骨肉瘤細胞
ATCC® Number: HTB-94™ Price: $338.00
Designations: SW 1353 [SW 1353, SW-1353]
Depositors: A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: fibroblast
HTB-94 SW 1353 人骨肉瘤細胞
Source: Organ: bone
Disease: chondrosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host （Roche FuGENE® Transfection Reagents）
Antigen Expression: Blood type O-
DNA Profile （STR）: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 10,11
D7S820: 9,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 2
Age: 72 years
Gender: female
Ethnicity: Caucasian
Comments: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.
The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.
A frozen ampule at passage 12 was received at the ATCC in January, 1982.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008
recommended serum:ATCC 30-2020
HTB-94 SW 1353
