96-square half deepwell microplate 225

96-square half deepwell microplate 225

96方半深孔微板孔內流體力學

對于96平方的半深孔微量滴定板（CR1496c，專門用于培養目的），通常使用0.25-0.35毫升的培養體積，在50毫米振幅的振動篩上。振蕩頻率取決于培養液的體積，對于0.25或0.3ml的培養液，一般采用225或250轉/分，這種微孔板的氧傳遞速率（OTR）尚未測定。從96平方深井板（Duetz等人，2000，應用和環境微生物學66:2641-2646）獲得的數據推斷，在225轉/分、40 mmol O2 l-1 h-1（對應于200 h-1的kLa值）下，估計的OTR為250 ul。這樣的OTR對應于在ErnMeYER振動篩燒瓶中的可實現（總瓶體積的培養體積10%，300轉/分），以確保*混合到底部（無死區），然而，建議使用250轉/分的振蕩頻率而不是225轉/分。在不使用高于0.25毫升的培養液的情況下（見本頁底部的兩個視頻蒸餾器。如果只有25毫米振幅的振動篩可用，則可以在培養液不接觸蓋子的情況下使用更高的振動頻率（275或300轉/分），還報告了在300轉/分（培養體積為0.25毫升）下搖動以獲得良好的結果（流體力學和氧轉移速率與500毫米/225轉/分相當）

建議（默認）條件：2～50毫米振動篩振幅，225轉/分鐘（大腸桿菌培養NB，20倍減慢）0.25毫升0.3毫升0.35毫升（*混合至0.30毫升培養體積）

 Hydrodynamics inside wells of a 96-square half deepwell microplate
For 96-square half deepwell microtiter plates (CR1496c, specifically designed for c*tion purposes), culture volumes between 0.25 ml and  0.35 ml are typically used, preferably on a shaker with a 50 mm amplitude. The optimal shaking frequency depends on the culture volume.For 0.25  or 0.3 ml cultures, 225 or 250 rpm is generally used.Oxygen transfer rates (OTR) for this kind of microplate have not been measured yet. Extrapolation from the data obtained with 96-square deepwell plates (Duetz et al., 2000, Applied and Environmental Microbiology 66:2641-2646) leads to an estimated OTR at 250 ul at 225 rpm of 40 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), To ensure full mixing down to the bottom (no dead zones), however, it may be advisable to use shaking frequencies of 250 rpm instead of 225 rpm. Even higher shaking frequencies (275 or 300 rpm) may be applied without the culture fluid touching upon the cover, on the condition that no higher culture volumes than 0.25 ml are used (see the bottom two video stills on this page.If only a shaker with a 25 mm amplitude is avaialble, shaking at 300 rpm (and a culture volume of 0.25 ml) is also reported to give good results (hydrodynamics and oxygen transfer rates comparable with 500 mm/225 rpm)
Adviced (default) condtion 2:50 mm shaker amplitude, 225 rpm(E. coli culture on NB, 20 times slowed down)    0.25 ml       0.3 ml      0.35 ml   (full mixing up to 0.30 ml culture volume)

 注：楊清辰發布