6-square deepwell microplate

6-square deepwell microplate

96平方深井孔板孔內流體力學

對于96平方深孔微量滴定板，通常使用0.5毫升至0.75毫升的培養體積，在50毫米振幅的振動篩上以300轉/分的速度進行培養（右圖為照片和視頻）。對于0.5毫升的培養物，這些振動條件導致38 mmol O2 l-1 h-1的氧轉移速率（OTR）（對應于200 h-1的kLa值）。這樣的OTR在其轉彎中對應于在ErnMEMEER振動篩燒瓶中的可實現（總瓶體積的培養體積10%，300轉/分），并且進一步確保混合到底部（無死區）。后者特別適用于大而重的細胞（如酵母菌和CHO細胞），這些細胞往往在死區沉淀。有些組使用0.6毫升的培養液，而不是0.5毫升，這仍然導致可接受的混合程度。

Hydrodynamics inside wells of a 96-square deepwell microplate
For 96-square deepwell microtiter plates, culture volumes between 0.5 ml and  0.75 ml are typically used, preferably at 300 rpm on a shaker with a 50 mm amplitude (photo and video on the right).For 0.5 ml cultures, these shaking conditions lead to oxygen transfer rates (OTR) of 38 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR - in its turn - corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), and furthermore ensures mixing down to the bottom (no dead zones). The latter is especially relevant  for large and heavy cells (such as yeasts and CHO-cells) that tend to precipitate in dead zones. Some groups use culture volumes of 0.6 ml instead of 0.5 ml, which still results in an acceptable degree of mixing.
25 mm shaker amplitude•300 rpm(full mixing up to 0.25 ml culture volume)

注：楊清辰發布