CartiGen 軟骨機械壓縮生物反應器系統
BISS TGT的生物反應器系列為三維組織工程構造提供機械刺激來創造體外生理條件。CartiGen系統賦予軟骨構建體壓縮或靜水應力（CartiGen HP）。應力/應變配置文件可以由用戶來定義，并且可以是一個簡單的諧波（正弦）或生理波形。

BISS TGT的生物反應器系列通過模擬生理狀態下的機械刺激條件來進行三維組織工程構建物的體外培養。CartiGen系統可壓縮/拉伸軟骨構建物壓縮。用戶可自定義應力/應變配置文件，實現簡單的調諧波（正弦）或生理波形。其應用領域包括干細胞的功能和分化、藥物測試、組織工程構建物的接種和生長、醫療器械的測試。目前研究人員正在利用這些系統在廣泛的范圍內開拓研究領域，包括：

• 干細胞刺激
• 半月板再生醫學
• 刺激軟骨細胞

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ProductsCartiGen: Mechanical Compression Bioreactor Systems

BISS TGT's Series of bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The CartiGen system imparts compressive or hydrostatic stress (CartiGen HP) to a cartilage construct. The stress/strain profiles can be defined by the user and can be a simple harmonic (sinusoidal) or a physiological waveform. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices. Researchers are currently utilizing these systems in a wide range of research areas including:

• Stem cell stimulation
• Meniscus regenerative medicine
• Chondrocyte stimulation

Mechanical Compression Bioreactor Configurations: 

BISS recognizes that research requirements can vary widely depending on the application and intended use.  We have a series of standard mechanical compression bioreactor system configurations to choose from.
 

Single Sample: C10-1

 

The C10-1 system has been designed as a single sample bioreactor system that is able to support a wide range of cell and tissue growth experiments utilizing compression. The chamber can accommodate a single sample with a maximum size of 30 mm diameter x 5 mm thick. Optional features such as additional mechanical transducers customize the instrument for the specific research application.

Multi-Sample & Imaging: C10-12c 

The C10-12c system has been designed as a multi-sample bioreactor system that is able to support a wide range of cell and tissue growth experiments. This chamber can accommodate up to 12 disc shaped samples with a maximum size of 10mm diameter x 5mm thick. The C10-12c's unique design includes optical grade windows on the bottom of the chamber, allowing researchers to view their samples with a confocol microscope without interrupting the culturing process. Optional features such as additional mechanical transducers customize the instrument for the specific research application.

Multi-Sample & Perfusion: C9-x 

The C9-x system has been designed as a multi-sample bioreactor system that is able to support a wide range of cell and tissue growth experiments. The chamber can accommodate up to 9 disc shaped samples with a maximum size of 8mm diameter x 5mm thick. The C9-x's unique design integrates porous platens within the bottom of the chamber to permit perfusion through the sample constructs. This feature allows researchers to direct flow through the construct while applying oscillatory compressive stimulation. Optional features such as additional mechanical transducers and perfusion systems customize the instrument for the specific research application.

 

Bioreactor Stimulator

Each of the CartiGen systems applies load using a 40N linear motor and monitored/controlled using an integrated load cell. The motor also incorporates a displacement transducer for monitoring and controlling the applied displacement. The motor delivers user defined waveforms of physiological significance.

GrowthWorks® Software & Control:

A fifth generation design, the GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.

The GrowthWorks Control Hardware features an embedded In CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.

CARTILAGE

Key: S=Standard, A=Available, - = not available

CHAMBERS	C1	C10-12c	C9-x
Individual sample growth chamber	S	S	S
Multi-sample growth chamber	-	S	S
Perfusion and instrumentation ports per chamber	-	-	9
Max construct size: 30 mm diameter by 5 mm thick	S	-	-
Max construct size: 10 mm diameter by 5 mm thick	S	S	-
Max construct size: 6 mm diameter by 5 mm thick	S	S	S
Sample visualization via confocal microscopy	-	S	-

 

STIMULATION	C1	C10-12c	C9-x
Axial stimulator and reaction frame	S	S	S
Compressed air and manifold	-	-	-

 

CONTROLS	C1	C10-12c	C9-x
Controller software, computer, and monitor	S	S	S
Power supplies and cabling	S	S	S
Mean flow control	A	-	S
Dynamic flow control	A	-	S

 

TISSUE MONITORING	C1	C10-12c	C9-x
Non-contact flow sensors	A	A	A
Axial load cell	S	S	S
Pressure transducer	-	-	-

 

ACCESSORIES	C1	C10-12c	C9-x
Media reservoir	S	-	S
Incubator	A	A	A

 

BENEFITS	C1	C10-12c	C9-x
All-autoclavable components	S	S	S
Medium change without opening chamber via luer fittings	-	-	S
Increased sterility with fast and clean sample change and access	S	S	S
Lightweight design for easy handling	S	S	S
Easily cleaned surface finishes	S	S	S
Compact design for fit in standard incubators 6.5cubic ft	S	S	S
* satisfaction guaranteed warranty	S	S	S