產(chǎn)品簡(jiǎn)介
產(chǎn)品英文名:全細(xì)胞亞硫酸鹽修飾試劑盒
產(chǎn)品英文名:Methylamp Whole Cell Bisulfite Modification Kit (40 samples)
*
40 samples/2002元 (貨號(hào):P-1016-40 )
80 samples/3738元 (貨號(hào):P-1016-80 )
品牌:美國(guó)epigentek
世聯(lián)博研(北京)科技有限公司 |
—— 銷售熱線 ——
13261877206 |
epigentek,P-1016,P-1016,P-1016-40,P-1016-80,*,
產(chǎn)品英文名:全細(xì)胞亞硫酸鹽修飾試劑盒
產(chǎn)品英文名:Methylamp Whole Cell Bisulfite Modification Kit (40 samples)
*
40 samples/2002元 (貨號(hào):P-1016-40 )
80 samples/3738元 (貨號(hào):P-1016-80 )
品牌:美國(guó)epigentek
The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
SCHEMATIC PROCEDURE: | |
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used. |
W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name. |
[Material Safety Data Sheet] |