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紅榮微再(上海)生物工程技術有限公司>>干細胞(無血清)培養基>>胚胎/多能干細胞培養基>> Cellartis人iPS誘導多能干細胞,搭配已馴化培養基

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人iPS誘導多能干細胞,搭配已馴化培養基

人iPS誘導多能干細胞,搭配已馴化培養基
參考價 22718
訂貨量 1
具體成交價以合同協議為準
  • 型號 Cellartis
  • 品牌 TaKaRa
  • 廠商性質 生產商
  • 產品資料 查看pdf文檔
  • 所在地 上海市

更新時間:2018-03-29 09:01:18瀏覽次數:2049

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產品簡介
供貨周期 一個月 規格 套裝
貨號 Y00185/Y00275/Y00285/Y00315/Y00325 主要用途 細胞治療、藥物監測和疾病模型等
人iPS誘導多能干細胞,搭配已馴化培養基,是一個完整的iPS誘導多能干細胞培養體系。
包括人iPS誘導多能干細胞和用于增殖培養的Cellartis DEF-CS Culture System培養體系。人皮膚細胞使用逆轉錄技術轉入Oct-4,SOX2,KLF4和c-Myc,所得iPS細胞表達Oct-4,NANOG,SSEA-3,SSEA-4,TRA-1 -60,TRA-1-81干細胞特異性標志物。

 華雅旗下紅榮微再授權代理(上海)TAKARA CellArtis,Rubicon產品 

1500 1904 520 紅榮微再-客服: 經銷商專員

產品圖片僅供參考。實際產品包括細胞株+培養基+添加劑+包被劑

人iPS誘導多能干細胞

(Human Induced Pluripotent Stem Cells)

 

產品英文名稱:Cellartis® Human iPS Cell Lines

產品中文名稱:Cellartis® 人iPS誘導多能干細胞

 

Cellartis人iPS細胞(Human iPS Cell Line)產品是達到了工業客戶使用標準生產的干細胞產品。該產品包括細胞和用于增殖培養的Cellartis DEF-CS Culture System培養體系。iPS細胞已經在制品化時完成了對DEF-CS培養體系的適應。Cellartis DEF-CS Culture System培養體系是組分明確而且不依賴飼養層的便捷的干細胞培養體系。Cellartis DEF-CS Culture System適合高效率地增殖培養干細胞,并且保持干細胞在增殖過程中幾乎無分化地生長,這使得不需要在大規模增殖培養過程中進行細胞篩選。因此,Cellartis人iPS細胞(Human iPS Cell Line)產品是一套完整的產品體系,對于科研級別和工業規模的需求都適合。

 

Cellartis人iPS誘導多能干細胞特點

細胞系通過使用缺陷型多順反子逆轉錄病毒(defective polycistronic retrovirus)技術重編程人皮膚成纖維細胞以轉入Oct-4,SOX2,KLF4和c-Myc

細胞系的純度和干細胞特征經過廣泛分析,包括解凍后的恢復,支原體和細菌檢測,干細胞特異性標記物的表達(Oct-4,NANOG,SSEA-3,SSEA-4,TRA-1 -60,TRA-1-81),不表達分化標記(β-3微管蛋白,FOXA2,ASMA)和預期核型

用Cellartis DEF-CS培養系統優化培養,試劑盒包括Cellartis DEF-CS 100培養系統

適用于所有主要應用; 細胞在分化時可以分化形成所有三個胚層的衍生物

Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc

Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype

Optimized for culture with the Cellartis DEF-CS Culture System

Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated

Kits include the Cellartis DEF-CS 100 Culture System

 

Cellartis 人iPS誘導多能干細胞參數

不同貨號對應供體年齡性別冷凍代數和已確認分化能力

 

 

1500 1904 520。紅榮微再-客服: 經銷商專員

iPS誘導多能干細胞的應用

細胞治療、藥物監測和疾病模型

 

Cellartis 人iPS誘導多能干細胞組份

不同供體的iPS細胞系和DEF-CS 100*培養基

*培養基包含基礎培養基、添加劑和包被劑。

保存:細胞 ≤ –150°C,添加劑 –20°C,基礎培養基和包被劑4 °C

 

A. Cellartis Human iPS Cell Lines

Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185)

Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225)

Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265)

Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275)

Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285)

Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305)

Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315)

Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325)

B. Cellartis DEF-CS 100 Culture System

· Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.)

o DEF-CS Basal Medium (100 ml)

o DEF-CS COAT-1 (for 100 ml) (800 µl)

o DEF-CS GF-1 (for 100 ml) (300 µl)

o DEF-CS GF-2 (for 100 ml) (100 µl)

o DEF-CS GF-3 (for 100 ml) (40 µl)

 

另行準備材料:PBS+TrypLE 選擇酶+TC處理ps表面培養器皿

The following materials are required but not supplied:

· PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)

· PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–)

· TrypLE Select Enzyme (1X), no phenol red

· Cell culture vessels, Tissue culture treated polystyrene surface

· General cell culture equipment used in cell culture laboratory

 

參考文獻:

1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.

2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.

3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.

4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.

 

Cellartis 人iPS誘導多能干細胞訂購信息

 

不同貨號的區別:iPS細胞供體不同

 

品牌

Code No.

包裝量

價格

Cellartis

Y00185

1 Kit

22718 元

Cellartis

Y00275

1 Kit

22718 元

Cellartis

Y00285

1 kit

22718 元

Cellartis

Y00315

1 Kit

22718 元

Cellartis

Y00325

1 kit

22718 元

Cellartis

Y00305

1 Kit

22718 元

 

關聯產品

人iPS細胞*培養基

http://www.om600.com/st321032/Product_29103798.html

 

華雅再生醫學旗艦公司:紅榮微再(上海)生物工程技術有限公司主營細胞治療和精準醫療等產品,2018年與TAKARA繼續合作,授權代理(上海)其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典,擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞,ips細胞,分化細胞等干細胞相關產品。

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