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Source

Mouse

Applications

WB

Reactivity

Species independent

Dilution

WB: 1:5000

Storage

-20°C/1 year

Specificity

The antibody detects KT3 tag fusion proteins.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Synthetic Peptide of KT3-Tag

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Background

The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. KT3 epitope tag can be used to monitor expression of the protein products transfected with cDNA constructs. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen.

Concentration

1 mg/ml