目錄:廈門逸漠生物科技有限公司>>培養產品>>蛋白>> IL-18重組蛋白
參考價 | ¥1200-¥5000 |
參考價:¥1200 ~ ¥5000
更新時間:2024-12-18 11:25:32瀏覽次數:263評價
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供貨周期 | 現貨 | 規格 | 10ug 20ug 50ug 100ug |
---|---|---|---|
貨號 | IMF-I18 |
IL-18重組蛋白
IL-18重組蛋白說明書
▍Backgound
Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, γδT cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressed IL-18 Rα which recruits IL-18 Rβ to form the signaling
receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs
▍Source
Recombinant Human IL-18 is expressed from Escherichia coli. It contains AA Tyr 37 - Asp 193. Predicted molecular mass 21.0 kDa. This protein carries a polyhistidine tag at the N-terminus.
▍Endotoxin
Less than 1.0 EU per μg by the LAL method.
▍AA Sequence
YFGK LESKLSVIRN LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI SVKCEKISTL SCENKIISFK EMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ FESSSYEGYF LACEKERDLF KLILKKEDEL GDRSIMFTVQ NED
▍Purity
Purity >90% as determined by SDS-PAGE.
▍Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
▍Reconstitution
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
▍Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
▍SDS-PAGE
Human IL-18, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.