目錄:廈門逸漠生物科技有限公司>>培養產品>>蛋白>> IL-1B重組蛋白
參考價 | ¥1200-¥5000 |
參考價:¥1200 ~ ¥5000
更新時間:2024-12-18 11:35:13瀏覽次數:467評價
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供貨周期 | 現貨 | 規格 | 10ug 20ug 50ug 100ug |
---|---|---|---|
貨號 | IMF-I01b |
IL-1B重組蛋白
IL-1B重組蛋白說明書
▍Backgound
Interleukin-1 beta (IL-1β, IL-1B) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.
▍Source
Recombinant Human IL-1B is expressed from Escherichia coli. It contains AA Ala 117 - Ser 269. Predicted molecular mass 20.7 kDa. This protein carries a polyhistidine tag at the N-terminus.
▍Endotoxin
Less than 1.0 EU per μg by the LAL method.
▍AA Sequence
APVRSLNCTL RDSQKSLVM SGPYELKALH LQGQDMEQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
▍Purity
Purity >90% as determined by SDS-PAGE.
▍Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
▍Reconstitution
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
▍Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
▍SDS-PAGE
Human IL-1 beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.