The PARP3 Chemiluminescent Activity Assay kit is designed to measure PARP3 activity for screening and profiling applications. The key to the PARP3 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with the assay buffer that contains the PARP3 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.