The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highlyspecific antibody that recognizes methylated substrate. With this kit, only three simplesteps on a microtiter plate are required for methyltransferase detection. First,Sadenosylmethionine is incubated with a sample containing assay buffer andmethyltransferase enzyme for one hour. Next, primary antibody is added. Finally, theplate is treated with an HRP-labeled secondary antibody followed by addition of the HRPsubstrate to produce chemiluminescence that can then be measured using achemiluminescence reader.