The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9aactivity for screening and profiling applications. The key to the G9a Direct Activity AssayKit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. Withthis kit, only three simple steps are required for methyltransferase detection. First,S-adenosylmethionine is incubated with a sample containing assay bufferａnd methyltransferase enzyme for one hour. Next, primary antibody is added.Finally, the strips are treated with an HRP-labeled secondary antibody followed byaddition of the HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.