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BPS Bioscience Inc.
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PDE3A TR-FRET Assay Kit

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更新時間:2023-10-11 22:37:25瀏覽次數:177次

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保存條件:-80°C英文名:PDE3ATR-FRETAssayKit數量:1供應商:BPSBioscienceInc
  • 保存條件:

    -80°C

  • 英文名:

    PDE3A TR-FRET Assay Kit

  • 數量:

    1

  • 供應商:

    BPS Bioscience Inc.

  • CAS號:

    /

  • 規格:

The PDE3A TR-FRET Assay Kit is designed to provide fast and easy identification of inhibitors of PDE3A (phosphodiesterase 3A) using Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET). The PDE3A TR-FRET Assay Kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PDE3A (amino acids 669-end) enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE assay buffer for 100 or 384 enzyme reactions.The reaction uses a fluorescein-conjugated (FAM) cyclic monophosphate nucleotide. Phosphodiesterase PDE3A catalyzes the hydrolysis of the phosphodiester bond in the cyclic monophosphate nucleotide, releasing the phosphate group for binding. The phosphate group binds to a "Binding Agent€ (BA) that is recognized by terbium-labeled donor beads. This results in energy transfer from the terbium to FAM, which emits a fluorescent signal at 520 nm. If unbound to the phosphate group, the terbium-labeled beads emit at λ=490 nm. The fluorescent intensity is measured using a fluorescence plate reader capable of TR-FRET reading and an increase in 520 nm corresponds directly to the activity of PDE3A.
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