The Myc Luciferase Reporter HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into the human colon cancer cell line HCT116. These cells contain a mutated APC gene, which leads to the aberrant accumulation of β-catenin and constitutive expression of Wnt-responsive genes by transcription factors TCF/LEF and CBP.

Transcription factor Myc is an early response gene that is over-expressed as a result of β-catenin signaling in HCT116 cells, leading to constitutive expression of the Myc-dependent luciferase reporter. The cell line is validated for inhibition of Myc activation by ICG-001, an inhibitor of β-catenin/CBP-mediated gene transcription.

Figure 1: Illustration of constitutive luciferase expression as a result of APC mutation and aberrant β-catenin signaling in Myc Luciferase Reporter HCT116 cells.
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