Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.