描述：
Image-iT Hypoxia Reagent是一種新型的、可固定的熒光標記物，專用于測定活細胞中的缺氧狀態。當活細胞處于正常氧濃度的環境中時，它是不發熒光的; 當氧濃度降低時，它的熒光亮度會變亮，可用于實時動態監測低氧下的細胞狀態。
可提供兩款缺氧檢測試劑 Image-iT™ Green Hypoxia Reagent (I14833, I14834) 和 Image-iT™ Red Hypoxia Reagent (H10498):
- 可動態監測：低氧環境下的活細胞；
- 兩種顏色可選： Image-iT™ Red Hypoxia 和 Image-iT™ Green Hypoxia；
- 可固定：Image-iT Green Hypoxia 可固定但不可逆,而Image-iT™ Red Hypoxia可進行缺氧可逆檢測；
- 簡便易用：直接加入培養細胞中即可成像；
Analyzing HeLa cells under hypoxic condition CellLight Histone 2B-RFP
HeLa cells were plated on MatTek dishes at a density of 100k/dish and incubated with CellLight Histone 2B-RFP (red) overnight at 37°C in CO2 incubator. Medium was replaced with fresh growth medium containing Image-iT Green Hypoxia Reagent at a final concentration of 5 µM. Cells were incubated at 20% or 1% oxygen for 3 hrs. The cells were then washed 2X with Live Cell Imaging Solution (LCIS) and incubated with and NucBlue Live nuclear stain (blue). Cells were imaged on an EVOS FL Auto Cell Imaging System.
Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Image-iT Green Hypoxia Reagent sustains its fluorescence when cells/tissue return to normal oxygen levels, allowing the cells/tissue to be fixed with minimal loss of fluorescent signal.
Features of Image-iT Green Hypoxia Reagent include:
? Indicates hypoxia in live cells by fluorescing in low oxygen environments
? Fixable—sustains fluorescent signal when cells/tissue return to normal oxygen levels
? Easy to use—just add to cell culture media and image
Image-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions. Image-iT Green Hypoxia Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT Green Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%. It responds quickly to such environments, and the fluorescence is sustained after the oxygen levels return to normal. These properties make Image-iT Green Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc.
For Research Use Only. Not for use in diagnostic procedures.
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