在2017年11月11日，由Fanni Li，Wenxi Dong，Peng Lin和Jianli Jiang在Biology of Reproduction中發表一篇了《Regulation of Akt/FoxO3a/Skp2 Axis Is Critically Involved in Berberine-Induced Cell Cycle Arrest in Hepatocellular Carcinoma Cells》的文章。

文章使用ECHO Revolve正倒置一體顯微鏡獲得了如下結果:

中文摘要：

ECHO Revolve正倒置一體顯微鏡觀察到的有序的細胞周期是腫瘤發生中關鍵的生物學過程，是抗癌藥物的重要靶點。小檗堿（BBR）是一種重要的天然異喹啉類生物堿，具有抗癌潛能，可誘導肝癌（HCC）細胞周期阻滯。然而，其基本機制尚待闡明。在我們目前研究中，觀察到小檗堿處理的HUH-7和HepG2細胞G0/G1細胞周期阻滯。簡單的說，BBR可使AKT通路失活，從而抑制S期激酶相關蛋白SKP2的表達，并增強FoxO3a在細胞核中的表達和轉運。易位的FXO3a一方面能直接促進細胞周期蛋白依賴性激酶抑制劑（CDKIS）p21^CIP1和p27^kip1的轉錄，另一方面抑制Skp2的表達，導致p21^CIP1和p27^kip1上調，肝癌HCC G0/G1細胞周期阻滯。綜上所述，BBR通過調節Akt/FoxO3A/Skp2促進CDKIs p21^CIP1和p27^kip1的表達，誘導肝癌HCC G0/G1細胞周期阻滯。本研究揭示了BBR抗癌作用的新機制。

英文摘要：

The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to

induce cell cycle arrest in hepatocellular carcinoma cells (HCC). However, the underlying mechanism remains to be elucidated. In our present study, G0/G1 phase cell cycle arrest was observed in berberine-treated Huh-7 and HepG2 cells. Mechanically, we observed that BBR could deactivate the Akt pathway, which consequently suppressed the S-phase kinase-associated protein 2 (Skp2) expression and enhanced the expression and translocation of Forkhead box O3a (FoxO3a) into nucleus. The translocated FoxO3a on one hand could directly promote the transcription of cyclin-dependent kinase inhibitors (CDKIs) p21^Cip1 and p27^Kip1, on the other hand, it could repress Skp2 expression, both of which lead to up-regulation of p21^Cip1 and p27^Kip1, causing G0/G1 phase cell cycle arrest in HCC. In conclusion, BBR promotes the expression of CDKIs p21^Cip1 and p27^Kip1 via regulating the Akt/FoxO3a/Skp2 axis and further induces HCC G0/G1 phase cell cycle arrest. This research uncovered a new mechanism of an anti-cancer effect of BBR. 

ECHO Revolve正倒置一體顯微鏡展現了其非凡的靈活性，可以輕松地實現正置和倒置顯微鏡轉換，創新性地把正倒置顯微鏡合二為一，開啟了顯微鏡Hybrid時代。

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ECHO Revolve正倒置一體顯微鏡兼具正置和倒置顯微鏡的優勢，方便小巧，一機多能，可以非常便利地通過旋轉實現正倒置配置切換；以iPad桌面替代目鏡設計, 獲得的視覺體驗；同時基于iOS的Echo app，使軟件操作更人性化。