D1045 5-Hydroxymethyl dCTP

品牌：ZYMO Research

代理商：慧穎生物

庫存：貨期產品

D1045 5-Hydroxymethyl dCTP

Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d^5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.

Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.

Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

About Hydroxy/Methylated Nucleotides

5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.

For 5-hmC

For 5-mC

Reagent

Volume

Final Concentration

Volume

Final Concentration

2X Reaction Buffer

25 µl

dNTP Mix

0.5 µl

0.25 mM each dNTP

0.5 µl

0.25 mM each dNTP

Forward Primer (10 µM)

Variable

0.3 to 1 µM

Variable

0.3 to 1 µM

Reverse Primer (10 µM)

Variable

0.3 to 1 µM

Variable

0.3 to 1 µM

Template DNA

Variable

< 200 ng/50 µl

Variable

< 200 ng/50 µl

ZymoTaq™ DNA Polymerase (5 U/µl)

1 µl

5 U/50 µl

0.4 µl

<2 U/50 µl

ddH₂0

to 50 µl

Total Volume

50 µl

Note: The final concentration of MgCl₂ in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl₂, primer, and/or template concentrations.
Specifications

Concentration	5-Hydroxymethylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d^5hmCTP). 5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution. 5-Methylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5mCTP). 5-Methyl dCTP: 1µmol/100 µl as a [10 mM] solution.
Applications	PCR amplicons containing 5-(hydroxy)methylcytosine are useful in a variety of applications such as: The study of 5-(hydroxy)methylcytosine binding/interacting protein Define methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.
Storage	5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.