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Invitrogen 18080-044 SuperScript? III Reverse Transcriptase 簡介：
產品名稱：SuperScript? III Reverse Transcriptase
中文名稱：反轉錄酶III
品牌：Invitrogen
貨號：18080-044
規格：10,000 units
市場價格：4245元
儲存溫度：-20°C
庫存狀態：現貨
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Invitrogen 18080-044 SuperScript? III Reverse Transcriptase 英文描述：
SuperScript? III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript? II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript? II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript? III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
? Thermostability – Half life of 220 minutes at 50°C
? Yield – Reduced RNase H activity for more full-length cDNA
? Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
? Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3′ and 5′ RACE
Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as a template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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