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產(chǎn)品名稱：Invitrogen 18005-017 T4 DNA 聚合酶
貨 號(hào)：18005-017
規(guī) 格：50U(5U/ul)
分 類： DNA/RNA 聚合酶
品 牌：Invitrogen
國(guó)產(chǎn)/進(jìn)口：進(jìn) 口
儲(chǔ)存溫度：-20°C
特惠價(jià)格：致 電
庫(kù)存狀態(tài)：現(xiàn) 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 18005-017 T4 DNA 聚合酶，常備現(xiàn)貨，*。主營(yíng)Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
Invitrogen 18005-017 T4 DNA 聚合酶描述：
T4 DNA Polymerase is a DNA polymerase that has a 3′-exodeoxyribonuclease activity, but lacks 5′a3′ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available.
Applications: Labeling double-stranded linear DNA by replacement synthesis (1). Oligonucleotide-directed, site-specific mutagenesis (2). 3′ end-labeling of double-stranded DNA (3). Polishing both 5′ or 3 overhangs to make blunt ends (4).
Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid.
Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template?primer.
Unit Reaction Conditions: 50 mM glycine-NaOH (pH 8.8), 16.6 mM (NH4)2SO4 , 6 mM MgCl2 , 6.5 μM EDTA, 10 mM 2-mercaptoethanol, 0.165 mg/ml BSA, 1.6 mg/ml DNase I-nicked salmon testes DNA, 0.33 mM dCTP, 0.33 mM dATP, 0.33 mM dGTP, 0.33 mM dTTP, 76 nM [3H]dTTP, and enzyme in 0.1 ml for 30 min. at 37°C.
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