softwellmatrigen 膠原蛋白包被的細(xì)胞培養(yǎng)皿--softwell產(chǎn)品
【簡(jiǎn)單介紹】
【詳細(xì)說(shuō)明】
柔軟的細(xì)胞培養(yǎng)板(皿),讓細(xì)胞不再睡在硬板床上
*,體內(nèi)細(xì)胞所處周邊環(huán)境的軟硬度 (softness,stiffness)可謂千差萬(wàn)別,可我們體外培養(yǎng)細(xì)胞時(shí)卻選擇了使用幾乎相同材質(zhì),硬邦邦的培養(yǎng)板(皿)。硬邦邦的培養(yǎng)板(皿)的硬度(E)達(dá)107kPa,而實(shí)際上,各組織細(xì)胞所處環(huán)境的硬度均低于100kPa,例如,E(小鼠胚胎干細(xì)胞)=0.6kPa,E(肝成纖維細(xì)胞)=1kPa,E(肝癌細(xì)胞)=1kPa,E(胚胎心肌細(xì)胞)=11kPa。可見(jiàn),給細(xì)胞一個(gè)類(lèi)似母體的舒適環(huán)境,更能正確的反映細(xì)胞生物學(xué)特征。
彈性指數(shù)(E)是指材質(zhì)的硬度(柔軟度),普通 培養(yǎng)板(皿) 的硬度要比身體組織硬105-109倍
Softwell 細(xì)胞培養(yǎng)板(皿)介紹
根據(jù)不同細(xì)胞類(lèi)型設(shè)計(jì)生產(chǎn)了硬度各異的透明水凝膠,并將這些水凝膠鋪設(shè)在我們常用細(xì)胞培養(yǎng)板、皿等用材的表面,薄薄的水凝膠讓您感覺(jué)不到它的存在,但細(xì)胞卻可以明顯感受到它的柔軟、舒適,從而讓細(xì)胞宛如生長(zhǎng)在母體的組織環(huán)境中。 例如可讓神經(jīng)細(xì)胞如同培養(yǎng)在腦組織中 ,讓心肌細(xì)胞如同在肌肉組織中生長(zhǎng),或者如同任何細(xì)胞的同源器官環(huán)境,從而讓您的實(shí)驗(yàn)數(shù)據(jù)更準(zhǔn)確。
Softwell系列產(chǎn)品表面水凝膠的優(yōu)點(diǎn):
1. 薄:只有幾毫米,肉眼幾乎看不到
2. 與培養(yǎng)板(皿)緊密結(jié)合:對(duì)胰酶消化、洗滌細(xì)胞、傳代均無(wú)影響
3. 透明:*光學(xué)穿透性,可用于顯微鏡觀測(cè)
4. 2D系統(tǒng):細(xì)胞生長(zhǎng)在凝膠表面,便于細(xì)胞分離、分析
5. 安全:對(duì)細(xì)胞無(wú)任何毒性
6. 穩(wěn)定:室溫下可保存6個(gè)月
Softwell系列產(chǎn)品優(yōu)勢(shì):
1. 適用于任何細(xì)胞,如神經(jīng)細(xì)胞、胚胎干細(xì)胞、心肌細(xì)胞等
2. 2D細(xì)胞培養(yǎng),卻能給細(xì)胞宛如母體的生長(zhǎng)環(huán)境
3. 可自行包被EMC蛋白或選擇包被好的產(chǎn)品
Softwell系列產(chǎn)品種類(lèi)很多,選擇方法如下:
1. 選擇不同功能的水凝膠,有三個(gè)系列
水凝膠功能 | ||
Easy coat 系列 | Collagen I 系列 | Non-Activated 系列 |
水凝膠已活化,可根據(jù)自身實(shí)驗(yàn)需要自行包被EMC蛋白,如膠原蛋白、纖連蛋白、層粘連蛋白等 | 水凝膠上已包被好I型膠原蛋白(取自鼠尾或綿羊皮),為即拆即用型,使用便捷 | 水凝膠未活化,其表面結(jié)合活性極低。用于懸浮細(xì)胞培養(yǎng)或自行活化 |
2. 選擇不同硬度的水凝膠
彈性指數(shù) (kPa) | ||
Soft (柔軟) | Intermediate (中度) | Stiff (硬) |
0.2 0.5 1 | 2 4 8 | 12 25 50 |
適用于培養(yǎng)脂肪、肝、肺、胚胎、脂肪、神經(jīng)部位的細(xì)胞 | 適用于培養(yǎng)肌肉、心臟部位的細(xì)胞 | 適用于培養(yǎng)骨骼部位的細(xì)胞 |
3. 選擇不同樣式的培養(yǎng)器皿
Softwell®系列
聚苯乙烯材質(zhì)的水凝膠培養(yǎng)板,規(guī)格有6孔、12孔、24孔、96孔
Softwell GTM 系列
黑色聚苯乙烯框架、硼硅玻璃孔徑的水凝膠培養(yǎng)板,規(guī)格有6孔、12孔、24孔、96孔、384孔
PetrisoftTM 系列
聚苯乙烯材質(zhì)的水凝膠培養(yǎng)皿,規(guī)格有35mm、100mm、150mm
SoftviewTM系列
35mm培養(yǎng)皿,里面放置覆蓋水凝膠的,高透度玻璃(直徑為10mm和20mm)
SoftslipTM 系列
內(nèi)含水凝膠玻璃蓋玻片的培養(yǎng)板,可取出蓋玻片觀察,規(guī)格有6孔、12孔、24孔
SoftslideTM 系列
內(nèi)含水凝膠的培養(yǎng)腔室玻片系統(tǒng),規(guī)格為16孔板
產(chǎn)品目錄如下
Softwell® 系列:聚苯乙烯材質(zhì)的水凝膠培養(yǎng)板
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
6孔板 | SW6-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-EC-HTS | Easy Coat | HTS |
6孔板 | SW6-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-COL-HTS | Collagen I | HTS |
6孔板 | SW6-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-NA-HTS | Non-Activated | HTS |
Softwell GTM 系列:黑色聚苯乙烯框架、硼硅玻璃孔徑的水凝膠培養(yǎng)板
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
6孔板 | SW6G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-EC-HTS | Easy Coat | HTS |
384孔板 | SW384G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-EC-HTS | Easy Coat | HTS |
6孔板 | SW6 G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-COL-HTS | Collagen I | HTS |
384 孔板 | SW384G-COL | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-COL-HTS | Easy Coat | HTS |
6孔板 | SW6G -NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-NA-HTS | Non-Activated | HTS |
384 孔板 | SW384G-NA | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-NA-HTS | Easy Coat | HTS |
PetrisoftTM系列:聚苯乙烯材質(zhì)的水凝膠培養(yǎng)皿
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
35mm培養(yǎng)皿 | PS35-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm培養(yǎng)皿 | PS100-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm培養(yǎng)皿 | PS150-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿 | PS35-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm 培養(yǎng)皿 | PS100-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm 培養(yǎng)皿 | PS150-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿 | PS35-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm 培養(yǎng)皿 | PS100-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm 培養(yǎng)皿 | PS150-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftviewTM系列: 35mm培養(yǎng)皿,里面放置覆蓋水凝膠的高透度玻璃
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
35mm 培養(yǎng)皿/10mm 玻片 | SV3510-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿/10mm玻片 | SV3510-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿/10mm玻片 | SV3510-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿/20mm玻片 | SV3520-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿/20mm玻片 | SV3520-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養(yǎng)皿/20mm玻片 | SV3520-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftslipTM系列:內(nèi)含水凝膠玻璃蓋玻片的培養(yǎng)板
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
6孔板/ 30 mm 蓋玻片 | SS6-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
6孔板/ 30 mm蓋玻片 | SS6-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
6孔板/ 30 mm蓋玻片 | SS6-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftslideTM系列:內(nèi)含水凝膠的培養(yǎng)腔室玻片系統(tǒng)
品名 | 貨號(hào) | 水凝膠功能 | 硬度(kpa) |
16 孔培養(yǎng)腔室 | SSL16-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
16 孔培養(yǎng)腔室 | SSL16-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
16 孔培養(yǎng)腔室 | SSL16-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
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