減數(shù)分裂重組蛋白SPO11抗體

英文名稱 SPO11
中文名稱 減數(shù)分裂重組蛋白SPO11抗體
別    名 Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN.

說 明 書 0.2ml  
研究領域 腫瘤  細胞生物  細胞周期蛋白  細胞分化  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Horse, 
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 45kDa
細胞定位 細胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human SPO11
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
Spo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility.

Function:
Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (By similarity). 

Subcellular Location:
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xy-12317R    SCUBE3新型分泌細胞表面蛋白/成骨細胞相關蛋白SCUBE3抗體
xy-12318R    SCUBE2內皮分泌蛋白SCUBE2抗體
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xy-10315R    phospho-Syntaxin 1a(Ser188)磷酸化突觸融合蛋白1抗體
xy-3787R    SCD1固醇酰輔酶A脫氫酶1抗體
xy-6015R    SNF2L解旋酶SNF2L抗體
xy-3829R    Sorbitol Dehydrogenase山梨醇脫氫酶抗體
xy-2381R    Signal recognition particle信號識別顆?？贵w
xy-6158R    SH2D3ASH2結構域蛋白3A抗體
xy-2943R    SEMA6D軸突導向因子SEMA6D抗體
xy-6531R    SKALP彈性蛋白酶抑制劑抗體
xy-2971R    STK39絲氨酸/蘇氨酸蛋白激酶39抗體
xy-5621R    phospho-STAT5a(Tyr694)磷酸化信號轉導和轉錄激活因子5a抗體
xy-5633R    phospho-SYT1(Thr202)磷酸化突觸結合蛋白1抗體
xy-5619R    phospho-STAT5a(Ser726)磷酸化信號轉導和轉錄激活因子5a抗體